Two floury outer endosperm mutants ( flo19-1 and flo19-2 ) were generated through editing LOC_Os03g48060 (named as FLO19 in this study), which encodes a class I glutamine amidotransferase. We identified a batch of potential chalky genes and prioritize one ( LOC_Os03g48060 ) for functional analyses. Here, we performed a genome-wide association study on chalkiness rate in a diverse panel of 533 cultivated rice accessions. Appearance quality and CEQ (cooking and eating quality) are two very important aspects in the evaluation of rice quality. Compared with ordinary rice, rice cultivars with superior eating quality and appearance quality are more popular with consumers due to its unique taste and ornamental value, even if their price is much higher. The combined effects of different SNPs of Wx are very important for rice quality breeding.Īs a staple food for more than half of the world’s population, the importance of rice is self-evident.

TT-AA-CC and TT-AA-TT had a low AAC and a soft GC. In1T-E圆A-Ex10C and In1T-E圆A-Ex10T were classified as being low AAC type. The combined effects of three SNPs had a significant influence on all ECQs and RVA profile parameters, except for gelatinization temperature. The apparent amylose content (AAC) was mainly controlled by the In1G/T SNP, and rice gel consistency (GC) was regulated by the Ex10C/T SNP. Had a dominant epistasis to SSIII-2 because the effect of SSIII-2 was masked by the massive synthesis of GBSS under Wx

, whereas there was a significant diversity under the same background of Wx There was no obvious difference between different SSIII-2 alleles under the same background of Wx The results revealed various effects of SSIII-2 on rice quality under different backgrounds of Wx alleles. The genotypes of SSIII-2 and the single nucleotide polymorphisms (SNPs) on intron1, exon6, and exon10 of Wx, and the physicochemical indicators and rapid visco analyzer (RVA) profile characteristics were analyzed. To investigate the effects of Wx and its interaction with SSIII-2 on grain ECQs, a population from a hybrid combination was established as a research material. SSIII-2 (SSIIIa), a member of the soluble starch synthases, is responsible for the synthesis of long chains of amylopectin. The Wx gene encodes a granule-bound starch synthase (GBSS) and plays a key role in determining rice eating and cooking qualities (ECQs).

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